TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristic(s) | Reference or source |
|---|---|---|
| S. aureus | ||
| RN4220 | 8325-4 r− | 16 |
| ISP794 | 8325-4 pig-131 | 34 |
| MT23142 | 8325 pig-131 flqB | 24 |
| KL820 | RN4220 norA::cat | 12 |
| QT1 | ISP794 mgrA::cat | 35 |
| QT5 | ISP794 norB::cat | This study |
| QT6 | ISP794 mgrA::cat norB::cat | This study |
| QT7 | ISP794 tet38Δ | This study |
| QT8 | ISP794 mgrA::cat tet38Δ | This study |
| E. coli DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA hsdR17(rK− mK−)supE44 λ−thi-1 gyrA96 relA1 | Gibco-BRL |
| Plasmids | ||
| pGEM3-zf(+) | 2.9-kb E. coli cloning vector, Apr | Promega |
| pLI50 | Shuttle cloning vector (Apr Cmr) | 32 |
| pCL52.2 | Temperature-sensitive E. coli-S. aureus shuttle vector | 32 |
| pSK950 | 10.5-kb plasmid carrying the attP site of phage L54a, replicon of pE194, Tcr Emr (S. aureus) | 25 |
| pQT8 | pSK950-norB | This study |
| pQT9 | pCL52.2-norB::cat | This study |
| pQT10 | pLI50-tet38 | This study |
| pQT11 | pCL52.2-tet38Δ | This study |