Table 3. Analytical-Scale Screening of Cosolvents for the Enzymatic Resolution of TBIM 2a Using E. coli Whole Cells Expressing MAO-N D11^a.

entry	solvent	time (h)	% eeb	yield (%)c
1	DMSO 5%	2	82	37
2		4	86	23
3		6	84	9
4	DMSO 10%	2	83	34
5		4	85	19
6		6	80	11
7	DMF 5%	2	94	44
8		4	94	26
9		6	94	18
10	MeOH 5%	2	68	41
11		4	82	23
12		6	80	15
13	PhMe 5%	2	68	40
14		4	60	23
15		6	68	12

^aConditions: cells 1 g/mL, phosphate buffer (0.1 M, pH 7), [syn-2a] = 4 mM, co-solvent 5–10%, BH₃NH₃, 37 °C.

^bCalculated by chiral HPLC.

^cCalculated by chiral HPLC using an external standard.