TABLE 2.
Germination of spores of various strains via the GerA receptora
| Strain | Genotype | CFU/12 h | Rate of DPA release (% of maximum value) |
|---|---|---|---|
| FB72 | ΔgerA ΔgerB ΔgerK | 1.5 × 105 | <2 |
| PS832 | 1.5 × 108 | 104 | |
| PS3611 | ΔgerB ΔgerK | 4.1 × 107 | 100b |
| PS3612 | gerACAla18 ΔgerB ΔgerK | 1.5 × 105 | <2 |
| PS3629 | ΔamyE::(PgerA-gerACCys18) gerACAla18 ΔgerB ΔgerK | 1.4 × 107 | 25 |
| PS3630 | ΔamyE::(PgerA-gerACAla18) ΔgerB ΔgerK | 4.7 × 107 | 104 |
| PS3631 | ΔamyE::(PsspB-gerACCys18) gerACAla18 ΔgerB ΔgerK | 1.5 × 108 | 360 |
| PS3632 | ΔamyE::(PsspB-gerACAla18) ΔgerB ΔgerK | 7 × 106 | 10 |
| PS3633 | ΔamyE::(PsspB-gerACCys18) ΔgerB ΔgerK | 470 | |
| PS3660 | ΔamyE::(PsspB-gerBCCys20) ΔgerB ΔgerK | 3.4 × 105 | 9 |
| PS3661 | ΔamyE::(PsspB-gerBCAla20) ΔgerB ΔgerK | 4.2 × 107 | 115 |
a
Spores were prepared, cleaned, and tested for colony-forming ability and for DPA release upon germination with l-alanine as described in the text. CFU values are the averages of duplicate determinations with two independent spore preparations; individual values differed by ≤25% from the average value. DPA release rates are the averages of duplicate measurements with two independent spore preparations; individual values differed by ≤25% from the average values.
b
This value was set at 100%.