TABLE 2.
Phenotypic switching of V. vulnificus 1003 phase variants
| Parent straina | Resulting phenotypes (avg % of total colonies)b
|
||
|---|---|---|---|
| Opaque (SE) | Translucent (SE) | Rugose (SE) | |
| 1003(O) | 55.2 (23.0) | 30.6 (16.2) | 13.0 (3.0) |
| BG(T1) | 17.3 (12.4) | 74.7 (15.9) | 5.8 (2.9) |
| BG(T2) | 1.7 (0.9) | 94.3 (2.1) | 3.8 (2.2) |
| AZ(T) | 0.5 (0.4) | 98.1 (1.7) | 0 (0.0) |
| ABZ1(T) | 0 (0.0) | 78.2 (16.0) | 18.2 (12.8) |
| GMB4(T) | 7.9 (7.7) | 79.8 (10.5) | 8.3 (5.5) |
| BG(R), 30°C | 15.8 (3.2) | 0.1 (0.04) | 53.8 (2.8) |
| BG(R), 37°C | 26.3 (15.5) | 0 (0.0) | 53.1 (21.8) |
| ABZ1(R), 30°C | 0 (0.0) | 42.9 (21.0) | 42.0 (13.0) |
| ABZ1(R), 37°C | 0 (0.0) | 3.7 (3.7) | 94.2 (5.0) |
a
O, opaque; T, translucent; R, rugose. Switching assays were conducted for one to three cycles of culture passaging and plating as described in the text, with O and T broth cultures incubated at 34 to 35°C and plates incubated at 30°C; R broth cultures and plates were incubated at either 30 or 37°C as indicated.
b
The total percentage of colonies for a given strain does not equal 100 because some colonies were sectored while others were of an intermediate phenotype.