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. 2023 Nov 2;14:1251452. doi: 10.3389/fimmu.2023.1251452

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Copyright © 2023 Pefanis, Bongoni, McRae, Salvaris, Fisicaro, Murphy, Ierino and Cowan

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Time course of pMLKL staining in the left kidney after IR in WT mice. Representative sections from 0 hrs (A), 3 hrs (B), 12 hrs (C), 24 hrs (D), 48 hrs (E), 72 hrs (F), and 4 weeks (G) post-reperfusion. (H): percentage of proximal tubules staining positive for pMLKL, stratified according to cellular location (dark blue = cytoplasmic; light blue = membrane/intraluminal). Examples of cytoplasmic, membrane and proteinaceous cast staining are indicated by arrowheads and short and long arrows, respectively. Kruskal-Wallis test with Dunn multiple comparisons test with * p<0.05, *** p<0.001 compared to baseline control. Image magnification x 400.