Table 1.

Primer and TaqMan probe sequences used to amplify bisulfite-converted DNA and RT-qPCR.

Gene	Forward primer sequence (5′-3′)	Reverse primer sequence (5′-3′)	Probe oligo sequence
MethyLight
FBXO43	TTTTAAAGTGGGAATGGGGAGAAGTAGAGT	CCCGCAAACCTAAATCCTCGCTTAAAC	CCTCTCTCGCTCACCCCTACACCCGTCCCG
β-Actin	TGGTGATGGAGGAGGTTTAGTAAGT	AACCAATAAAACCTACTCCTCCCTTAAA	ACCACCACCCAACACACAATAACAAACACA
RT-qPCR
FBXO43	GGAAAGTAAGCAGAAATTGGCGTG	GAGTGGCAGCATCCTCGACATT
β-Actin	ATGGGTCAGAAGGATTCCTATGTG	CTTCATGAGGTAGTCAGTCAGGTC

RT-qPCR, quantitative real-time PCR; FBXO43, F-box protein 43.