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Principle used to distinguish between 5′ (A) and 3′ (B) overhangs of the cohesive ends. The sequence of the cohesive ends would appear in both end-fragment sequences if the phage has 5′ overhangs, while it would appear in neither sequence in the case of 3′ protrusion. End-fragment sequences were compared to the sequence of the covalently closed cos site carried by pPAG165.
