TABLE 1.
Plasmids used in the studies
| Plasmid | Relevant features | Reference |
|---|---|---|
| pRK2013 | Helper in conjugative transfer of pBBR1MCS-5 derivative plasmids | 15 |
| pCU101 | Helper in conjugative transfer of plasmid with pCU1 mob | 33 |
| pBBR1MCS-5 | Derivative of the broad-host-range cloning vector pBBR1MCS; Gmr; replicate in Escherichia coli and R. meliloti | 19 |
| pDH31 | Cosmid clone, contains the 25-kb 87EcoRI-41EcoRI region of phage 16-3 | 11 |
| pCU999 | Source of kanamycin cassette | 24 |
| pCU996Ω | Source of spectinomycin cassette (Ω) | 2 |
| pSEM155 | pSC101 replicon, pCU1 mob, attP, Kmr | 14 |
| pPAG160 | attP was deleted from pSEM155, and the Km cassette was replaced by the spectinomycin cassette, derived from pCU996Ω | This work |
| pPAG165 | 96BglII-14EcoRI fragment (nt 1-5006) cloned in pPAG160 | This work |
| pPAG168 | pPAG165 derivative, Km cassette replaced codons 34-81 in ORF525 | This work |
| pPAG169 | pPAG165 derivative, Km cassette inserted at codon 59 in ORF154 | This work |
| pPAG185 | pPAG165 derivative, Km cassette inserted at codon 56 in ORF171 | This work |
| pPAG201 | Similar to pPAG165 but contains the 6-kb 94BamHI-14EcoRI region and the Km cassette replaced codons 60-75 in ORF249 | This work |
| pCS473 | pBBR1MCS-5 containing the region from nt 1650-2466 including ORF154 | This work |
| pCS474 | pBBR1MCS-5 containing from nt 1823-3984 including ORF525 | This work |
| pCS475 | pBBR1MCS-5 containing from nt 1-932 including ORF249 | This work |