TABLE 4.
PCR amplification of ORFs from shared regions of divergence and adjacent flanking ORFs
| Region | ORF | Observation of PCR productb
|
||||||
|---|---|---|---|---|---|---|---|---|
| 6006 | 6058 | 6004 | 6010 | MAA104 | 19698 | K10 | ||
| —a | MAP0092 | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| MAP_RD1 | MAP0096c | − | − | − | + | + | +++ | +++ |
| MAP_RD1 | MAP0106c | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| MAP0107 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | |
| MAP0845 | +++ | +++ | +++ | +++ | + | +++ | +++ | |
| MAP_RD2 | MAP0850c | +++ | +++ | +++ | + | + | +++ | +++ |
| MAP_RD2 | MAP0866 | − | − | + | + | +++ | +++ | +++ |
| MAP0867c | +++ | +++ | +++ | +++ | +++ | +++ | +++ | |
| MAP2146c | + | + | + | + | + | + | + | |
| MAP_RD3 | MAP2158 | + | + | + | + | + | +++ | +++ |
| MAP2164 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | |
| MAP_RD4 | MAP2189 | + | + | + | + | +++ | +++ | +++ |
| MAP2198 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | |
| MAP2749c | +++ | +++ | +++ | +++ | +++ | +++ | +++ | |
| MAP_RD5 | MAP2756c | − | − | − | + | + | +++ | +++ |
| MAP_RD5 | MAP2765c | − | − | − | + | +++ | +++ | +++ |
| MAP2770 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | |
| MAP3715 | − | + | − | − | − | +++ | +++ | |
| MAP_RD6 | MAP3726 | − | − | − | + | + | +++ | +++ |
| MAP_RD6 | MAP3751 | + | − | + | + | +++ | +++ | +++ |
| MAP_RD6 | MAP3775c | − | − | − | + | + | +++ | +++ |
| MAP3783 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | |
a
—, ORF adjacent to divergent region and predicted to be present by microarray hybridization.
b
+++, product observed; −, no product observed; +, weak product observed.