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. 2005 Apr;187(7):2439–2447. doi: 10.1128/JB.187.7.2439-2447.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 1. — Inactivation of rel_Msm in M. smegmatis. (A) Thin-layer chromatography of radiolabeled (p)ppGpp. E. coli strain JM105 (lane 1) was induced for the stringent response by d,l-serine hydroxamate (lane 2). The appearance of labeled (p)ppGpp is indicated with an arrowhead. Wild-type M. smegmatis (lane 3) and M. smegmatis Δrel_Msm (lane 4) were induced for the stringent response by d,l-norvaline (lanes 5 and 6, respectively). Radiolabeled (p)ppGpp is present in lane 5 (wild-type strain) but absent from lane 6 (Δrel_Msm strain). (B) Agarose gel electrophoresis of RNAs obtained from either wild-type M. smegmatis (lane 1) or M. smegmatis Δrel_Msm (lane 2) cells that had been grown to stationary phase (3-day-old cultures). A 1-kb DNA ladder is present in lane L. rRNA bands are indicated (arrowheads).