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. 2005 Apr;187(7):2439–2447. doi: 10.1128/JB.187.7.2439-2447.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 5. — Regulation of eis (Rv2416c) by mycobacterial stringent response. (A) M. tuberculosis strains H37Rv and H37Rv Δrel_Mtb were grown from early log (lanes 1 and 2) to mid-log (lanes 3 to 6) and stationary (lanes 7 to 9) phase. Protein lysates were equilibrated (22 μg/lane) and examined for eis expression by Western blot analysis. (B) Coomassie-stained proteins (25 μg/lane) are shown for M. smegmatis/pOLYG (lane 1), M. smegmatis Δrel_Msm/pOLYG (lane 2), M. smegmatis/p69 (lane 3), and M. smegmatis Δrel_Msm/p69 (lane 4). (C) Western blot analysis of Eis with the same protein lysates as those used for panel B.