TABLE 2.

Oligonucleotides used for PCR experiments

Primer	Oligonucleotide sequence (5′ → 3′)	PCR product size (bp)	Use
yfgL1	ATGATGCAGATGAAAATTAATAATTTGTCCATCTGA	1,148	ΔyfgL isogenic mutant construction
	GAGGGACCCGAAAGCCACGTTGTGTCTCAA
yfgL2	CGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACG
	ACAGAGACGAGCGCTGAGGTCTGCCTCGTG
A2GBL-3	AAAGCCACGTTGTGTCTCAA	957	Kanamycin resistance cassette amplification
B2GBLnp5	TTAGAAAAACTCATCGAGCA
yfgL3	GTAGTGCATGGGAAGCAGGC	1,378	Isogenic mutant verification
yfgL4	TAAATCATCAGACAACGCACGC
yfgL5	CGTGGAGCACTTCCGTTGG	941	Isogenic mutant verification
yfgL6	GCTGGGCAACGAAACGACC
yfgLXbaI	CCCGGGTCTAGATCCATCTGAGAGGGACCCGATGC	1,263	Cloning of yfgL
yfgLPstl	CTGCAGCGGCCCCTGTCCAGGAGCCG