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. 2023 Nov 16;16:421. doi: 10.1186/s13071-023-06024-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Effect of PfK13 mutation or iron supplementation on the reproduction of Plasmodium falciparum 3D7. A The workflow involved monitoring growth curves, conducting in vitro iron sucrose assays, and comparing hemozoin content. B Comparison of merozoites and their number between P. falciparum 3D7^WT and P. falciparum 3D7^C580Y. C Impact of iron supplementation on merozoites and their number in P. falciparum 3D7^C580Y. D Effect of iron supplementation on the number of merozoites of P. falciparum 3D7^WT and P. falciparum 3D7^C580Y. E Comparison of hemozoin content between P. falciparum 3D7^WT and P. falciparum 3D7^C580Y. F Comparison of growth curves between P. falciparum 3D7^WT and P. falciparum 3D7^C580Y. G Flow-cytometry analysis showing the impact of desferrioxamine (DFO) on the growth and development of P. falciparum 3D7. ns No significant difference (P > 0.05), **P < 0.01, ****P < 0.0001 (t-test)