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. 2005 Mar 14;33(5):1532–1543. doi: 10.1093/nar/gki293

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Full-length extension of primer hybridized to modified templates. Reactions were carried out at 30°C for 20 min using a natural template (X = T) (lanes 1 and 2) or modified templates containing one unnatural base X (X = 7-Ni), sequence 10 [lanes 3 and 4) or 5-Ni, sequence 11 (lanes 5 and 6); 100 nM] primed with ³²P-labeled 18mer (sequence 12, 50 nM) in the presence of the four dNTPs (at a final concentration of 20 μM each). Reactions were catalyzed by KF exo− (0.1 U).