Table 1.
The P.chrysogenum BIBAC libraries and their clones used in the physical map construction
| Features | HindIII library | BamHI library | Total |
|---|---|---|---|
| Cloning vector | pCLD04541 | pCLD04541 | 1 |
| Cloning sites | HindIII | BamHI | 2 |
| No. of clones | 4608 | 3456 | 8064 |
| Average insert size (kb) | 130 | 126 | 128 |
| Insert-empty clones (%) | 0.0 | 4.1 | 1.8 |
| Genome equivalents | 17.3× | 12.1× | 29.4× |
| No. of clones fingerprinted | 4224 | 3072 | 7296 |
| No. of clone fingerprints used | 3849 | 2315 | 6164 |
| Genome equivalents used | 14.5× | 8.6× | 23.1× |