Table 2.

Contig assembly from the fingerprints of 6164 BIBACs (23.1×) generated with single enzyme or labeling colors, or the combinations of multiple enzymes or labeling colors by lowering the cutoff settings until ≤1% Qs were generated

Combinationa of color(s)	No. of bands per clone	Size represented per band (bp)	Cutoff needed for 1% Qs	No. of contigs	No. of clones in the contigs	No. of singles	No. of Qs	Mean length/contig (kb)b
B	34.4	3721	5 × 10−25	216	5344	820	15	231
G	37.8	3386	1 × 10−24	213	4521	1642	21	293
Y	26.0	4923	1 × 10−29	161	5294	867	46	278
R	40.2	3184	1 × 10−23	89	5851	313	4	426
Mean	34.6	3699		170	5252	910	22	307
BG	73.0	1753	1 × 10−30	246	5040	1124	36	245
BR	74.6	1716	1 × 10−27	42	5969	195	44	758
BY	60.4	2119	2 × 10−28	98	5744	420	28	406
RY	66.2	1934	5 × 10−28	66	5824	340	19	553
GR	78.9	1622	5 × 10−32	255	5176	988	41	236
GY	64.7	1978	1 × 10−28	211	4897	1267	40	270
Mean	69.6	1839		153	5441	722	35	411
BGR	113.2	1131	1 × 10−35	299	5373	791	48	216
BGY	99.0	1293	1 × 10−35	395	4934	1230	29	196
BRY	100.6	1272	3 × 10−30	65	5913	251	32	564
GRY	104.9	1220	1 × 10−35	340	5152	1012	22	209
Mean	104.4	1226		275	5343	821	33	296
BGYR	139.3	919	1 × 10−35	281	5439	725	30	225

^aLetter ‘B’ indicates ‘blue’ fingerprints generated with BamHI; ‘G’ for ‘green’ fingerprints generated with HindIII; ‘Y’ for ‘yellow’ fingerprints generated with XbaI; and ‘R’ for ‘red’ fingerprints generated with XhoI.

^bThe mean length of the contigs from each assembly was calculated by the product of the mean size that each unique band (the average insert size of the clones divided by the average number of bands per clone) is equivalent to and the total number of unique bands of all contigs divided by the number of contigs of the assembly.