Table 2.
List of PCR primers (5′→3′); one of the inner primers is biotin labeled
| SNP | Outer forward primer | Outer reverse primer | Inner forward primer | Inner reverse primer |
|---|---|---|---|---|
| 1 | ctcccagaagaggggtgg | cgtgtgtgcacggcctgt | gggtagcccctggagacc | cacggcctgtgtcctgtcctct |
| 2 | tccatgccacagacctcaat | cttgttgtactcgctgatgg | cctcaatgacaagagtgtgca | tactcgctgatggcaaagtccag |
| 3 | tgaaattaacctcagtgagg | tgctccccactactctcaga | agtgaggtgaggagacaatta | ccactactctcagaaacttcttgaa |
| 4 | acctgggggctaaagaagaa | agggacgcatcttgctgtg | gaagaagagctgtctctggg | tcttgctgtgtacgggaggagg |
| 5 | gatgcctgggggtgtgaag | tagcagaggaaggagtggc | tgaagaaaagagccttgggtt | aaggagtggccccaggttccc |
| 6 | agaactactctgtcaaagct | gcaaggctgggctcccaag | gctcccgctgagggtgg | gggctcccaagaaagcactgac |
| 7 | ctttgacttggttgagcgata | caaccatttgccaggcaagg | gcgatagggaaagtgttagc | gccaggcaaggaagcaccggg |
| 8 | ctcagcagtgtcatgatgaa | gataaggctagctaccagtg | tgatgaaatgagtgtgctgac | ctagctaccagtgtccaccacac |
| 9 | ccctcctaagtggcagcca | cagtgctcacctcctgtgg | gccatgtgctccataaccc | ctcctgtggcggttgtgcgga |
| 10 | agctctgcagcgtgtcgc | gctgagcccggtgggtgg | gccctcaagctgtaggcc | ggtgggtgggtggagggactg |
| 11 | tgctccgctccttccctcc | actgcatgaccacccggag | cctcctctgtaaaatgggga | gaccacccggagacataagtact |
| 12 | gactgctgttaggagatttc | tacaggcaggttaaagacca | gatttctcccagataggctc | gcaggttaaagaccaaaacatttcaa |
| 13 | gggtcacattctggcttaag | aggagagacacatgggggc | cattctggcttaagcgccaatttc | tgggggcagaggggacgggaaa |