Fig 4.

 Gel mobility shift assay. (A) Effect of paeoniflorin (PF). Lane 1, extract from control cells; lane 2, extract from heat-shocked (42°C for 2 hours) cells; lanes 3, 4, 5, 6, and 7, extracts from cells treated with 80 μg/mL of paeoniflorin for 4, 8, 12, 16, and 24 hours, respectively; lane 8, extract from cells heat shocked (42°C for 2 hours) and then incubated with anti–heat shock transcription factor 1 (HSF1) antibody; lane 9, extract from cells treated with 80 μg/mL of paeoniflorin for 4 hours and then incubated with anti-HSF1 antibody; lane 10, extract from cells treated with 80 μg/mL of paeoniflorin for 8 hours and then incubated with anti-HSF1 antibody. (B) Effect of glycyrrhizin (GL). Lane 1, extract from control cells; lane 2, extract from heat-shocked (42°C for 2 hours) cells; lanes 3, 4, 5, 6, and 7, extracts from cells treated with 80 μg/mL glycyrrhizin for 4, 8, 12, 16, and 24 hours, respectively; lane 8, extract from cells heat shocked (42°C for 2 hours) and then incubated with anti-HSF1 antibody; lane 9, extract from cells treated with 80 μg/mL of glycyrrhizin for 4 hours and then incubated with anti-HSF1 antibody; lane 10, extract from cells treated with 80 μg/mL of glycyrrhizin for 8 hours and then incubated with anti-HSF1 antibody. Open arrowheads indicate nonspecific bands. Filled arrowheads indicate heat shock element–protein complexes. Arrows indicate supershifted complexes with anti-HSF1 antibody