Fig 6.

 (A) Development of thermotolerance in HeLa cells treated with paeoniflorin and the 960 mixture (containing paeoniflorin). Cells were treated with heat shock (42°C for 2 hours, ▵), 960 mixture (400 μg/mL for 24 hours, ⋄), or paeoniflorin (PF, 80 μg/mL for 24 hours, □) and then heated at 45°C for the indicated period. After 45°C heating, cells were subjected to the clonogenic survival assay. (B) Effect of glycyrrhizin and the 941 mixture (not containing paeoniflorin) on the development of thermotolerance in HeLa cells. Cells were treated with heat shock (42°C for 2 hours, ▴), 941 mixture (400 μg/mL for 24 hours, ♦), or glycyrrhizin (GL, 80 μg/mL for 24 hours, ▪) and then heated at 45°C for the indicated period. After 45°C heating, cells were subjected to the clonogenic survival assay. Survival curves of nontreated control cells are indicated by open and closed circles in panel A and panel B, respectively. All data points represent the means of 3 independent experiments