Fig 2.

 Regulation of pIE1 and pIE2 enhancers is independent of the basal promoter. T-47D (A) or A549 (B) cells were transfected with luciferase-reporter plasmids driven by the FKBP5 promoter (p531), the pIE1 enhancer upstream of the FKBP5 promoter (pIE1h51), the pIE1 enhancer in pGL3-Promoter (pIE1), the pIE2 enhancer upstream of the FKBP5 promoter (pIE2h51), or the pIE2 enhancer in pGL3-Promoter (pIE2). T-47D cells (A) were treated with vehicle or 10-nM R5020, whereas A549 cells (B) were treated with vehicle or 10-nM dexamethasone for 24 hours, after which the cells were collected for assay of luciferase activity. The data are expressed as fold induction over untreated cells. Each bar represents the mean ± SEM of at least 3 independent experiments