Figure 1.

Construction and structural features of the PP13138R vaccine. The PP13138R vaccine was designed with TLR-2 agonist PorB, helper peptide PADRE, HTL epitopes, CTL epitopes, and B-cell epitopes, which are highlighted in different colors in (A) and depicted in their corresponding amino acid sequences in (B). The secondary structure of the PP13138R vaccine was predicted using the Prabi server and is shown in (C), where the alpha helix is illustrated in blue, the extended strand in red, and the random coil in purple. The transmembrane region (D) and signal peptide (E) of the PP13138R vaccine were predicted using the NovoPro server. Inside represents the intracellular region, and higher values indicate a higher likelihood that the amino acid resides within this area. Outside indicates the extracellular region, where next to it is a higher probability that the amino acid exists outside of the cell membrane. The transmembrane signifies the transmembrane section and greater values indicate a higher probability of the amino acid being present in this region.