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. 2023 Nov 2;14:1280299. doi: 10.3389/fimmu.2023.1280299

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Copyright © 2023 Jiang, Han, Liu, Xue, Cheng, Xiao and Gong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction of pET28a-PP13138R recombinant plasmid and expression and purification of the PP13138R vaccine in vitro. The codon-optimized gene sequence of the PP13138R vaccine was inserted into the genome of the pET28a plasmid via NheI and XhoI double digestion sites to construct the pET28a-PP13138R recombinant plasmid (A). The recombinant plasmid was introduced into the E. coli expression vector to express the PP13138R vaccine, purified by 6× His-tag adsorption on a nickel column, and finally identified by SDS-PAGE (B).