FIG. 2.

Effect of nutritional factors on AflR (AFLR) expression. A. parasiticus NRRL 2999 was grown in various media (PMS, GMS, GMS−Zn, GMS+NH₄NO₃, and GMS+NaNO₃) for 70 h. Duplicate samples in each group were collected, subjected to sodium dodecyl sulfate–10% polyacrylamide gel electrophoresis (50 μg of protein/lane), and tested by Western blot analysis with anti-AflR antibodies. Results of the Western blot analysis for AflR are shown in the top panel. The accumulation of AFB₁ and relative AflR levels in Western blots are shown in the bottom panel. The intensity of the AflR signal for the GMS medium culture was considered 100%.