FIG. 3.

Kinetics of aflR and omtA expression in A. parasiticus following a nutritional shift. A. parasiticus NRRL 3386 was first grown in PMS medium for 48 h and then transferred to GMS medium and grown for another 4 to 42 h under the same incubation conditions. Fungal extracts and RNA of duplicate samples were isolated before transfer (0 h) and at the indicated times following transfer. Fungal extracts were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (60 μg of protein/lane) and then Western blot analysis with anti-AflR (AFLR) antibodies. RNA samples at 25 and 8 μg were subjected to RPA with either aflR cRNA and an 18S rRNA riboprobe or omtA cRNA and an 18S rRNA riboprobe as the probes, respectively. The results are shown in the top panel. The accumulation of AFB₁ is shown in the bottom panel.