FIG. 4.
Expression of aflR and omtA in selected nonaflatoxigenic species. Eight fungal species were first grown in PMS medium for 64 h and then distributed to GMS medium and grown for another 24 h. Levels of AflR (AFLR) and of aflR and omtA transcripts were determined as described in the legend to Fig. 3. Lanes 1′ and 1, A. parasiticus NRRL 3386; lane 2, A. sojae NRRL 5596; lanes 3′ and 3, A. flavus NRRL 3357; lane 4, A. flavus SRRC 2111; lane 5, A. flavus NRRL 6341; lane 6, A. flavus NRRL 482; lane 7, A. oryzae NRRL 451; lane 8, A. oryzae NRRL 1911. Samples in lanes 1′ and 3′ were collected at transfer; others were collected after being transferred to GMS medium and grown for 24 h. The results are shown in the top panel. The accumulation of AFB1 in the culture filtrate and O-MTase activity are shown in the bottom panel.