FIG. 6.

Western blot analysis of AflR in fungal extracts of various species. Various fungal species were grown in SLS medium at 29°C for 70 h. Protein extracts from these cultures were prepared, subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (60 μg of protein/lane), and tested by Western blot analysis with anti-AflR antibodies. Lane 1, A. flavus NRRL 5565; lane 2, A. nomius NRRL 3161; lane 3, A. versicolor SRRC 111; lane 4, A. nidulans FGSC 26; lane 5, A. sojae NRRL 6271; lane 6, C. sativus ATCC 24081. Molecular masses for the marker proteins are indicated.