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. 2023 Oct 18;91(11):e00355-23. doi: 10.1128/iai.00355-23

TABLE 2.

Organ agar hits

Gene Name New locus tag Plate a Well a OD600 Organ b Function General function PCR confirm c
PMI0003 thrC PMI_RS00015 216 G4 0.862 U Threonine synthase Amino acid metabolism n/a
PMI0005 PMI_RS00025 213 F6 0.880 U Conserved hypothetical protein n/a
PMI0006 talB PMI_RS00030 213 G2 0.818 UK Transaldolase B Carbohydrate metabolism N
PMI0083 nusB PMI_RS00400 210 C11 0.333 K N utilization substance protein B Transcription machinery n/a
PMI0200 dksA PMI_RS00965 211 F9 0.877 K DnaK suppressor protein rRNA transcription factor n/a
PMI0205 hemL PMI_RS00985 219 C2 0.500 UK Glutamate-1-semialdehyde 2,1-aminomutase Amino acid metabolism Y
PMI0206 erpA PMI_RS00990 211 E9 0.576 UK Putative iron-sulfur protein Y
PMI0335 proC PMI_RS01600 202 G2 0.644 U Pyrroline-5-carboxylate reductase Amino acid metabolism n/a
PMI0370 proA PMI_RS01770 208 E11 0.727 U Gamma-glutamyl phosphate reductase Amino acid metabolism n/a
PMI0570 d sucB PMI_RS02805 207 F6 0.863 UKL Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex TCA cycle e Y
PMI0641 sanA PMI_RS03160 215 C10 0.854 K Putative transport protein Y
PMI0711 serC PMI_RS03500 219 C4 0.419 UK Phosphoserine aminotransferase Amino acid metabolism Y
PMI0765 ompF/ nmpC PMI_RS03760 218 G6 0.716 UK Outer membrane porin Signal transduction Y
PMI1151 znuC PMI_RS05555 201 G4 0.775 KL High-affinity zinc uptake system ATP-binding protein Transport Y
PMI1152 znuA PMI_RS05560 209 C1 0.990 K High-affinity zinc uptake system substrate-binding protein Transport n/a
PMI1344 trpD PMI_RS06485 204 B8 0.914 K Anthranilate synthase component (glutamine amidotransferase) Amino acid metabolism n/a
PMI1348 trpA PMI_RS06505 204 C4 0.889 UK Tryptophan synthase alpha chain Amino acid metabolism n/a
PMI1545 guaA PMI_RS07520 201 D12 0.807 UKL GMP synthase (glutamine-hydrolyzing) Nucleotide metabolism Y
PMI1562 purC PMI_RS07610 209 G4 0.880 U Phosphoribosylaminoimidazole-succinocarboxamide synthetase (SAICAR synthetase) Nucleotide metabolism n/a
PMI1833 cysW PMI_RS09045 201 A5 0.609 K Sulfate/thiosulfate ABC transporter, permease protein Transport Y
PMI1875 purL PMI_RS09255 201 C11 0.737 U Phosphoribosylformylglycineamide synthetase Nucleotide metabolism n/a
PMI1912 PMI_RS09435 207 F3 0.737 U FtsK/SpoIIIE-family protein Chromosome partitioning n/a
PMI1945 ireA PMI_RS09585 202 A9 0.714 U Putative TonB-dependent ferric siderophore receptor Transport n/a
PMI2085 leuB PMI_RS10270 207 C5 0.946 U 3-Isopropylmalate dehydrogenase Amino acid metabolism n/a
PMI2094 speA PMI_RS10315 201 H1 0.571 K Biosynthetic arginine decarboxylase Amino acid metabolism n/a
PMI2288 dapD PMI_RS11300 210 E12 0.746 K 2,3,4,5-Tetrahydropyridine-2-carboxylate N-succinyltransferase Amino acid metabolism n/a
PMI2309 recB PMI_RS11420 219 B3 0.241 UK Exodeoxyribonuclease V beta chain DNA repair n/a
PMI2720 PMI_RS13405 213 D5 0.771 U BMC domain-containing protein n/a
PMI2821 argD PMI_RS13935 210 A4 0.720 U Acetylornithine/succinyldiaminopimelate aminotransferase Amino acid metabolism n/a
PMI2870 PMI_RS14185 208 F4 0.575 UKL Hypothetical protein Y
PMI2930 glpD/ glyD PMI_RS14485 210 D8 0.727 K Aerobic glycerol-3-phosphate dehydrogenase Lipid metabolism n/a
PMI3027 aroB PMI_RS14975 219 A3 0.734 U 3-Dehydroquinate synthase Amino acid metabolism n/a
PMI3028 PMI_RS14980 213 F2 0.946 U SPOR domain-containing protein Peptidoglycan binding n/a
PMI3175 waaF/ rfaF PMI_RS15700 219 B2 0.402 K ADP-heptose--LPS heptosyltransferase II Lipopolysaccharide biosynthesis n/a
PMI3180 envC PMI_RS15725 211 E2 0.564 K Putative exported peptidase/murein hydrolase activator EnvC Cell division Y
PMI3185 cysE PMI_RS15750 212 G7 0.954 UL Serine acetyltransferase Amino acid metabolism n/a
PMI3210 glpK PMI_RS15875 214 E2 0.958 U Glycerol kinase Lipid metabolism Y
PMI3236 argE PMI_RS16000 209 F6 0.812 U Acetylornithine deacetylase Amino acid metabolism n/a
PMI3301 ilvE PMI_RS16410 207 B4 1.016 U Branched-chain amino acid aminotransferase Amino acid metabolism Y
PMI3302 ilvD PMI_RS16415 207 D2 0.913 U Dihydroxy-acid dehydratase Amino acid metabolism n/a
PMI3316 wecA/ rfe PMI_RS16480 219 D6 0.351 U Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase Lipopolysaccharide biosynthesis Y
PMI3319 rffD PMI_RS16495 219 C5 0.398 K UDP-N-acetyl-d-mannosamine dehydrogenase Carbohydrate metabolism n/a
PMI3384 fklB PMI_RS16845 213 E11 0.588 K FkbP-type 22 kDa peptidyl-prolyl cis-trans isomerase Chaperones and folding catalysts N
PMI3431 PMI_RS17100 213 E9 0.974 U Two-component system sensor kinase Signaling and cellular processes N
PMI3457 argI PMI_RS17230 204 D1 0.911 UL Ornithine carbamoyltransferase chain I Amino acid metabolism Y
PMI3528 metR PMI_RS17535 201 A2 0.728 U LysR-family transcriptional regulator Amino acid metabolism Y
PMI3538 ubiB PMI_RS17585 206 A11 0.939 U Probable ubiquinone biosynthesis protein Cofactor metabolism N
PMIt068 PMI_RS16125 219 C1 0.490 K tRNA Transcription machinery n/a
a

Location of transposon mutants in the ordered library.

b

Urine, Kidney, Liver.

c

The computationally predicted location of the transposon was tested by PCR for 20 mutants.

d

Seven PCR-confirmed mutants that were selected for follow-up studies are highlighted in bold.

e

sucB, primarily known as a component of the TCA cycle, is also involved in amino acid metabolism; it was included as a metabolic gene for purposes of categorization in Fig. 2.