TABLE 2.
Gene | Name | New locus tag | Plate a | Well a | OD600 | Organ b | Function | General function | PCR confirm c |
---|---|---|---|---|---|---|---|---|---|
PMI0003 | thrC | PMI_RS00015 | 216 | G4 | 0.862 | U | Threonine synthase | Amino acid metabolism | n/a |
PMI0005 | PMI_RS00025 | 213 | F6 | 0.880 | U | Conserved hypothetical protein | n/a | ||
PMI0006 | talB | PMI_RS00030 | 213 | G2 | 0.818 | UK | Transaldolase B | Carbohydrate metabolism | N |
PMI0083 | nusB | PMI_RS00400 | 210 | C11 | 0.333 | K | N utilization substance protein B | Transcription machinery | n/a |
PMI0200 | dksA | PMI_RS00965 | 211 | F9 | 0.877 | K | DnaK suppressor protein | rRNA transcription factor | n/a |
PMI0205 | hemL | PMI_RS00985 | 219 | C2 | 0.500 | UK | Glutamate-1-semialdehyde 2,1-aminomutase | Amino acid metabolism | Y |
PMI0206 | erpA | PMI_RS00990 | 211 | E9 | 0.576 | UK | Putative iron-sulfur protein | Y | |
PMI0335 | proC | PMI_RS01600 | 202 | G2 | 0.644 | U | Pyrroline-5-carboxylate reductase | Amino acid metabolism | n/a |
PMI0370 | proA | PMI_RS01770 | 208 | E11 | 0.727 | U | Gamma-glutamyl phosphate reductase | Amino acid metabolism | n/a |
PMI0570 d | sucB | PMI_RS02805 | 207 | F6 | 0.863 | UKL | Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex | TCA cycle e | Y |
PMI0641 | sanA | PMI_RS03160 | 215 | C10 | 0.854 | K | Putative transport protein | Y | |
PMI0711 | serC | PMI_RS03500 | 219 | C4 | 0.419 | UK | Phosphoserine aminotransferase | Amino acid metabolism | Y |
PMI0765 | ompF/ nmpC | PMI_RS03760 | 218 | G6 | 0.716 | UK | Outer membrane porin | Signal transduction | Y |
PMI1151 | znuC | PMI_RS05555 | 201 | G4 | 0.775 | KL | High-affinity zinc uptake system ATP-binding protein | Transport | Y |
PMI1152 | znuA | PMI_RS05560 | 209 | C1 | 0.990 | K | High-affinity zinc uptake system substrate-binding protein | Transport | n/a |
PMI1344 | trpD | PMI_RS06485 | 204 | B8 | 0.914 | K | Anthranilate synthase component (glutamine amidotransferase) | Amino acid metabolism | n/a |
PMI1348 | trpA | PMI_RS06505 | 204 | C4 | 0.889 | UK | Tryptophan synthase alpha chain | Amino acid metabolism | n/a |
PMI1545 | guaA | PMI_RS07520 | 201 | D12 | 0.807 | UKL | GMP synthase (glutamine-hydrolyzing) | Nucleotide metabolism | Y |
PMI1562 | purC | PMI_RS07610 | 209 | G4 | 0.880 | U | Phosphoribosylaminoimidazole-succinocarboxamide synthetase (SAICAR synthetase) | Nucleotide metabolism | n/a |
PMI1833 | cysW | PMI_RS09045 | 201 | A5 | 0.609 | K | Sulfate/thiosulfate ABC transporter, permease protein | Transport | Y |
PMI1875 | purL | PMI_RS09255 | 201 | C11 | 0.737 | U | Phosphoribosylformylglycineamide synthetase | Nucleotide metabolism | n/a |
PMI1912 | PMI_RS09435 | 207 | F3 | 0.737 | U | FtsK/SpoIIIE-family protein | Chromosome partitioning | n/a | |
PMI1945 | ireA | PMI_RS09585 | 202 | A9 | 0.714 | U | Putative TonB-dependent ferric siderophore receptor | Transport | n/a |
PMI2085 | leuB | PMI_RS10270 | 207 | C5 | 0.946 | U | 3-Isopropylmalate dehydrogenase | Amino acid metabolism | n/a |
PMI2094 | speA | PMI_RS10315 | 201 | H1 | 0.571 | K | Biosynthetic arginine decarboxylase | Amino acid metabolism | n/a |
PMI2288 | dapD | PMI_RS11300 | 210 | E12 | 0.746 | K | 2,3,4,5-Tetrahydropyridine-2-carboxylate N-succinyltransferase | Amino acid metabolism | n/a |
PMI2309 | recB | PMI_RS11420 | 219 | B3 | 0.241 | UK | Exodeoxyribonuclease V beta chain | DNA repair | n/a |
PMI2720 | PMI_RS13405 | 213 | D5 | 0.771 | U | BMC domain-containing protein | n/a | ||
PMI2821 | argD | PMI_RS13935 | 210 | A4 | 0.720 | U | Acetylornithine/succinyldiaminopimelate aminotransferase | Amino acid metabolism | n/a |
PMI2870 | PMI_RS14185 | 208 | F4 | 0.575 | UKL | Hypothetical protein | Y | ||
PMI2930 | glpD/ glyD | PMI_RS14485 | 210 | D8 | 0.727 | K | Aerobic glycerol-3-phosphate dehydrogenase | Lipid metabolism | n/a |
PMI3027 | aroB | PMI_RS14975 | 219 | A3 | 0.734 | U | 3-Dehydroquinate synthase | Amino acid metabolism | n/a |
PMI3028 | PMI_RS14980 | 213 | F2 | 0.946 | U | SPOR domain-containing protein | Peptidoglycan binding | n/a | |
PMI3175 | waaF/ rfaF | PMI_RS15700 | 219 | B2 | 0.402 | K | ADP-heptose--LPS heptosyltransferase II | Lipopolysaccharide biosynthesis | n/a |
PMI3180 | envC | PMI_RS15725 | 211 | E2 | 0.564 | K | Putative exported peptidase/murein hydrolase activator EnvC | Cell division | Y |
PMI3185 | cysE | PMI_RS15750 | 212 | G7 | 0.954 | UL | Serine acetyltransferase | Amino acid metabolism | n/a |
PMI3210 | glpK | PMI_RS15875 | 214 | E2 | 0.958 | U | Glycerol kinase | Lipid metabolism | Y |
PMI3236 | argE | PMI_RS16000 | 209 | F6 | 0.812 | U | Acetylornithine deacetylase | Amino acid metabolism | n/a |
PMI3301 | ilvE | PMI_RS16410 | 207 | B4 | 1.016 | U | Branched-chain amino acid aminotransferase | Amino acid metabolism | Y |
PMI3302 | ilvD | PMI_RS16415 | 207 | D2 | 0.913 | U | Dihydroxy-acid dehydratase | Amino acid metabolism | n/a |
PMI3316 | wecA/ rfe | PMI_RS16480 | 219 | D6 | 0.351 | U | Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase | Lipopolysaccharide biosynthesis | Y |
PMI3319 | rffD | PMI_RS16495 | 219 | C5 | 0.398 | K | UDP-N-acetyl-d-mannosamine dehydrogenase | Carbohydrate metabolism | n/a |
PMI3384 | fklB | PMI_RS16845 | 213 | E11 | 0.588 | K | FkbP-type 22 kDa peptidyl-prolyl cis-trans isomerase | Chaperones and folding catalysts | N |
PMI3431 | PMI_RS17100 | 213 | E9 | 0.974 | U | Two-component system sensor kinase | Signaling and cellular processes | N | |
PMI3457 | argI | PMI_RS17230 | 204 | D1 | 0.911 | UL | Ornithine carbamoyltransferase chain I | Amino acid metabolism | Y |
PMI3528 | metR | PMI_RS17535 | 201 | A2 | 0.728 | U | LysR-family transcriptional regulator | Amino acid metabolism | Y |
PMI3538 | ubiB | PMI_RS17585 | 206 | A11 | 0.939 | U | Probable ubiquinone biosynthesis protein | Cofactor metabolism | N |
PMIt068 | PMI_RS16125 | 219 | C1 | 0.490 | K | tRNA | Transcription machinery | n/a |
Location of transposon mutants in the ordered library.
Urine, Kidney, Liver.
The computationally predicted location of the transposon was tested by PCR for 20 mutants.
Seven PCR-confirmed mutants that were selected for follow-up studies are highlighted in bold.
sucB, primarily known as a component of the TCA cycle, is also involved in amino acid metabolism; it was included as a metabolic gene for purposes of categorization in Fig. 2.