Fig 1.

Effect of heat shock protein 27 (HSP27) on floating fibroblast-populated collagen lattice (FPCL) contraction. (A) Expression of HSP27. Western blot analysis of whole-cell lysates from STO cells transfected with a rat HSP27 sense-strand construct (SS12), with vector only (VO) and with an anti-sense strand HSP27 construct (AS10). (B) Growth curves of 3 lines. Increased cell numbers of SS12 cells (black circles), VO cells (open triangles), and AS10 cells (gray squares) were monitored over 4 days in culture. (C) Floating FPCL contraction. Contraction of floating FPCL populated with SS12 cells (black columns), VO cells (white columns), or AS10 cells (gray columns) was measured at 24 hours with or without treatment with lisophospatidic acid (20 μM) or platelet-derived growth factor BB (100 ng/mL) in the presence and absence of 15 μM Y-27632. *, P < 0.05 vs VO by Student's t-test. +, P < 0.05 vs value of the same cell without Y-27632