Fig 2.

Effect of heat shock protein 27 (HSP27) on cellular elongation in a collagen matrix. (A) Images from time-lapse movies of cells plated in collagen matrix. Images were taken 8 hours and 16 hours after plating. Bar = 100 μm. (B) Photomicrographs of cells in collagen lattices cultured for 24 hours after plating in collagen in a humidified incubator with a 5% CO₂–95% air atmosphere. Bar = 100 μm. (C) The average length of the longer axis of 60 SS12, VO, and AS10 cells after 24 hours of culture in collagen. Error bars represent standard error of the mean (SEM). *, P < 0.05 vs VO by Student's t-test. (D) The average ratios of the longer axis vs shorter axis of 60 SS12, VO, and AS10 cells after 24 hours of culture in collagen. Error bars represent SEM. *, P < 0.05 vs VO by Student's t-test