Fig 1.

Induction of messenger ribonucleic acid (mRNAs) for molecular chaperone in the carbon tetrachloride (CCl₄)–treated rat liver. (A) Rats were given intraperitoneally various amounts of CCl₄, as indicated on the top. Total RNA was isolated from the rat liver at indicated times after CCl₄ injection. RNAs (2.25 μg) were subjected to blot analysis for Hsc70, Hsp70, dj2, and dj3 mRNAs as described in the Materials and Methods. Control RNA was total RNA from vehicle-treated rat liver. The positions of 28S ribosomal RNA (rRNA) are shown on the right. The bottom panel shows ethidium bromide staining of 28S rRNAs. (B) Serum ALT activities were measured at indicated times after CCl₄ (1.0 mL/kg) injection, and the results are shown as means ± SE (n = 3). ALT, alanine aminotransferase; Hsc70, heat shock cognate 70; Hsp70, heat shock protein 70