TABLE 3.

Effect of primer degeneracies on PCR product ratios^a

Prepn	Eco(GC)/Eco(AT)m ratiob
	15 cycles	25 cycles	35 cycles
Template mixture	1.0 ± 0.04	1.0 ± 0.04	1.0 ± 0.04
PCR 1	1.4 ± 0.16	1.5 ± 0.06	2.3 ± 0.10
PCR 2	1.4 ± 0.14	1.8 ± 0.11	2.3 ± 0.07
PCR 3	1.3 ± 0.11	1.7 ± 0.07	2.5 ± 0.20
PCR 4	1.4 ± 0.26	1.9 ± 0.07	1.9 ± 0.14
PCR 5	1.3 ± 0.16	1.8 ± 0.09	2.0 ± 0.17

^aThe data were generated by dot blot hybridization by using the template-specific probes Eco and EcoM and mixtures containing equal amounts of templates Eco(GC) and Eco(AT)m. PCR products were amplified from the same template mixtures for 15, 25, and 35 cycles. 

^bMeans ± standard deviations were calculated from data for three replicate dots for each of the samples which was hybridized with the specific probes and quantified by phosphorimaging.