TABLE 4.

Reproducibility of PCR amplification of a single template in a complex mixture of nucleic acids from a natural community^a

Prepn	V. anguillarum/(eu)bacterial 16S rDNA ratiob
	10% V. anguillarum	1% V. anguillarum	0.1% V. anguillarum
PCR 1	0.156 ± 0.008	0.017 ± 0.001	NDc
PCR 2	0.147 ± 0.011	0.020 ± 0.000	ND
PCR 3	0.153 ± 0.005	0.016 ± 0.001	ND

^aThe data were generated by dot blot hybridization by using the V. anguillarum-specific probe Van219 and the universal (eu)bacterial probe S-D-Bact-0338-a-A-18. PCR products were amplified from nucleic acids extracted from a natural community to which known amounts of V. anguillarum DNA were added. 

^bMeans ± standard deviations were calculated from data for three replicate dots for each of the samples which was hybridized with the specific probes and quantified by phosphorimaging. 

^cND, not detected (below detection limit).