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Refolding activity of heat shock proteins. Activity is expressed as a percentage of renaturation after heating at 42°C compared with unheated control samples. The different proteins, rbHSC70, rhHSP70, and mHSP70, were analyzed using 300 μg/mL of each and sampled at the times indicated. The mHSP70 corresponds to the protein purified from skeletal muscle containing both Hsp70 and Hsc70. Rabbit reticulocyte lysate, RL, was used as a positive control for the luciferase assay system
