TABLE A1.

Enzymatic biosensing at a glance.

Authors	Biorecognition	Analyte	Linear range	Limit of detection	Notes	Refs.
Photoluminescent and colorimetric biosensors
Li et al.	Horseradish Peroxidase	Hydroquinone	0.24–9.6 μM	84 nM	On/Off Sensor	[151]
Xiaoyan et al.	Horseradish Peroxidase	H2O2	2 nM–200 μM	0.2 nM	On/Off Sensor	[152]
Caballero-Diaz et al.	Acetylcholinesterase	Fenoxycarb	6–70 μM	3.15 μM	Inhibitor Based	[153]
Sahub et al.	Choline Oxidase	Dichlorvos	0.45–45.25 μM	0.172 ppm/0.778 μM	Inhibitor Based	[154]
Li et al.	Acetylcholinesterase	Paraxon	0.001–1.0 μg/mL	0.4 ng/mL	Ratiometric	[155]
Kong et al.	α-Glucosidase	Activity; Acarbose	0–10 U/mL; 100 nM–1 mM	0.01 U/mL; 10 nM	Inhibitor Based	[156]
Huang et al.	Alkaline Phosphatase	Activity; Na3VO4	0.02–0.08 U/mL; 0–1 mM	0.008 U/mL; –	Inhibitor Based	[157]
Cho et al.	Glucose Oxidase	Glucose	0.1–500 μM	40 nM	Ratiometric	[158]
Li et al.	Oxidoreductases	Variable	Variable	Variable	General System	[159]
Tang et al.	Tyrosinase	Dopamine	0.1–60 μM	60 nM	Conjugated	[160]
Bui and Park	Hemoglobin	Cholesterol	0–800 μM	56 μM	On/Off Sensor	[161]
Bhunia et al.	Hemoglobin	H2O2	0–100 mM	-	On/Off Sensor	[162]
Kong et al.	Uricase	Uric Acid	5–500 μM	2 μM	On/Off Sensor	[163]
Shao et al.	Urease	Urea	0.1–100 mM	0.01 mM	On/Off Sensor	[164]
Shi et al.	Glucose Oxidase	Glucose	1 μM–0.5 mM	0.4 μM	Nanozyme	[117]
Wang et al.	Glucose Oxidase	Glucose	0–100 μM	20 μM	Nanozyme	[118]
Zhou et al.	Pyranose Oxidase	1,5-Anhydroglucitol	20–100 μg/mL	144 ng/mL	Nanozyme	[165]
Lin et al.	Glucose Oxidase	Glucose	25–375 μM	5.3 μM	Nanozyme	[166]
Wang et al.	Glucose Oxidase	Glucose	0.2–50 μM	0.2 μM	Nanozyme	[167]
Zhong et al.	Glucose Oxidase	Glucose	0.01–2 mM	3 μM	Nanozyme	[168]
Honarasa et al.	Glucose Oxidase	Glucose	0.7–300 mμM	0.7 μM	Nanozyme	[169]
Electrochemical biosensors
Vasilescu et al.	Laccase	Caffeic Acid	380 nM–100 μM	320 nM	Amperometric	[170]
Razmi et al.	Glucose Oxidase	Glucose	5–60 μM	1.73 μM	Amperometric/DET	[171]
Muthurasu et al.	Horseradish Peroxidase	H2O2	0.1–1.3 mM; 1.7–2.6 mM	530 nM	Amperometric/DET	[172]
Wang et al.	Horseradish Peroxidase	H2O2	0.1–23.1 μM	40 nM	Amperometric/DET	[173]
Zhao et al.	Glucose Oxidase	Glucose	0–64 mM	1.07 μM	Amperometric/DET	[174]
Gupta et al.	Many	Many	10 μM–3 mM (Glucose)	1.35 μM	Amperometric/DET	[175]
Baluta et al.	Laccase	Epinephrine	1–120 μM	83 nM	Amperometric/DET	[176]
Chemiluminescent biosensors
Hassanzadeh et al.	Cholesterol Oxidase	Cholesterol	80 nM–30 μM	35 nM	Nanozyme	[177]
Photoelectrochemical biosensors
Cheng et al.	Acetylcholinesterase	Acetylcholine	1 ng/mL–1.5 μg/mL	70 pg/mL	Visible Light	[178]