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Representative Scatchard plot of [3H]CGS21680 saturation binding data. Empty circles indicate neutrophil membranes from healthy volunteers (affinity constant [Kd] = 25 ± 1.3 nmol/l; maximum number of binding sites [Bmax] = 35 ± 2.4 fmol/mg); filled circles indicate neutrophil membranes from systemic sclerosis (SSc) patients overall (Kd = 23 ± 1.8 nmol/l; Bmax = 40 ± 3.2 fmol/mg). Assays were performed in triplicate.
