Figure 5.
A2B adenosine receptor (AR)-mediated stimulation of adenylyl cyclase activity in control (empty circles) and systemic sclerosis (SSc; filled circles) neutrophil membranes. Membranes were incubated with different NECA concentrations (ranging from 10 nmol/l to 100 μmol/l) and the activity of adenylyl cyclase, expressed as pmol/min per mg protein, was evaluated. Values are expressed as mean ± standard error of three indipendent experiments. EC50 values were 165 ± 9.3 for control versus 373 ± 26 nmol/l for SSc.