Extended Data Figure 10.

(related to Figure 6). Top: DMS-MaPseq reactivity for IFRD2 intron 9 (grey shade) +/− 10 nucleotides in the in vitro transcribed RNAs shown above. Individual dots represent two biological replicates. Bottom: Heatmap showing the log2 fold change of DMS reactivity in U2_SE or del_int4–8 relative to WT. Positions corresponding to G’s and T’s, which do not react with DMS, are shown in light grey. Positions where the largest fold change is observed in both mutated constructs are shown in orange font. Predicted branch points^45,60 are shown as brown rectangles and the two predicted SRSF1 binding sites (ESEFinder⁴⁶) with the highest scores are shown as beige rectangles.