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Effects of various metal ions on ability of hyaluronic acid (HA) to inhibit the activity of antithrombin (AT). HA (1000 μg/ml) and antithrombin (150 μg/ml) were incubated with various concentrations of CaCl2, FeCl3, KCl, MgCl2, or NaCl. Thrombin activity of blank (reaction buffer only) was considered as 1 and the activities of other tests were normalized based on comparisons with blank. Values are expressed as mean ± standard deviation of data from triplicate experiments.
