Table 1.
Primers for detection of murine FcγRI, II, and III mRNA
| Gene | Primer | Primer sequence |
| GAPDH | Up | 5'-GGC-AAA-TTC-AAC-GGC-ACA-3' |
| Low | 5'-GTT-AGT-GGG-GTC-TCG-CTC-CTG-3' | |
| FcγRI | Up | 5'-ACA-CAA-TGG-TTT-ATC-AAC-GGA-ACA-3' |
| Low | 5'-TGG-CCT-CTG-GGA-TGC-TAT-AAC-T-3' | |
| FcγRII | Up | 5'-GAC-AGC-CGT-GCT-AAA-TCT-TGC-T-3' |
| Low | 5'-GTG-TCA-CCG-TGT-CTT-CCT-TGA-G-3' | |
| FcγRIII | Up | 5'-GAC-AGG-CAG-AGT-GCA-GCT-CTT-3' |
| Low | 5'-TGT-CTT-CCT-TGA-GCA-CCT-GGA-T-3' | |
| MMP-3 | Up | 5'-TGG-AGC-TGA-TGC-ATA-AGC-CC-3' |
| Low | 5'-TGA-AGC-CAC-CAA-CAT-CAG-GA-3' | |
| MMP-9 | Up | 5'-GGA-ACT-CAC-ACG-ACA-TCT-TCC-A-3' |
| Low | 5'-GAA-ACT-CAC-ACG-CCA-GAA-GAA-TTT-3' | |
| MMP-12 | Up | 5'-GGA-CAT-GAA-GCG-TGA-GGA-TGT-3' |
| Low | 5'-GAA-GTC-TCC-GTG-AGC-TCC-AAA-T-3' | |
| MMP-13 | Up | 5'-ACC-TTG-TGT-TTG-CAG-AGC-ACT-AAC-TT-3' |
| Low | 5'-CTT-CAG-GAT-TCC-CGC-AAG-AGT-3' | |
| MMP-14 | Up | 5'-AAG-GCT-GAT-TTG-GCA-ACC-AT-3' |
| Low | 5'-GTC-CCA-AAC-TTA-TCC-GGA-ACA-C-3' |
Primer sequences used for RT-PCR on synovium. FcγR, Fcγ receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP, matrix metalloproteinase.