Table 3.

Effect of IL-13 on MMP mRNA levels in synovium of mice with ICA

	ICA day 3		ICA day 7
	AxCANI	AxCAhIL-13	AxCANI	AxCAhIL-13
MMP-3	5.7 ± 0.3	7.1 ± 0.8	4.2 ± 0.5	6.1 ± 0.2*
MMP-9	5.1 ± 0.2	4.8 ± 0.3	0.2 ± 0.6	3.9 ± 0.5*
MMP-12	0.6 ± 0.4	5.8 ± 1.1*	0.9 ± 1	8.1 ± 0.9*
MMP-13	3.2 ± 0.2	2.7 ± 0.3	4.3 ± 0.3	6.4 ± 0.3*
MMP-14	3.7 ± 0.4	4.9 ± 0.8	3.7 ± 1	3.7 ± 0.6

Expression profile of MMP-3, -9, -12, -13, and -14 mRNA levels after injection of AxCANI or AxCAhIL-13 in synovial biopsies isolated at day 3 and day 7 after arthritis onset. The Ct values for MMP genes in naive knee joints were subtracted from the Ct values for MMPs at day 3 and 7 after arthritis onset. Ct values were corrected for GAPDH content for each individual sample. Note that MMP-3, -9, -12, and -13 mRNA levels were significantly increased at day 7 by IL-13, and the MMP-12 mRNA level was already elevated at day 3. Values represent means ± SEM for 5 mice. *P < 0.05, Mann–Whitney U test. AxCAhIL-13 = adenovirus encoding interleukin-13; AxCANI = adenovirus encoding no gene; Ct = cycle threshold; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; ICA = immune-complex-mediated arthritis; MMP = matrix metalloproteinase.