Table II.
Overview of the identified metal transporters in C. reinhardtii
Columns 1 to 6 contain protein name, JGI identification number (transcript), scaffold number (version 2 of the C. reinhardtii genome), protein model length, predicted subcellular localization, and number of EST clones found. Subcellular localization is indicated when both prediction programs (iPSORT and TargetP) agree. Organelle localization corresponds to either chloroplast or mitochondria, and secretory pathway corresponds to ER, Golgi, or plasma membrane localization. For more details, see Supplemental Tables II, IV, and VI. CrMRP1 (AAL35383), CrCds1 (AAQ19847), and CrFTR1 (AAM45938) have been named previously (Im and Grossman, 2002; La Fontaine et al., 2002; Hanikenne et al., 2005). –, Not found.
| Protein Name | JGI ID | Scaffold | Length (Amino Acids) | Predicted Subcellular Localization | No. of EST Clones |
|---|---|---|---|---|---|
| CDF Family | |||||
| CrMTP1 | 168519 | 67 | 640 | Vacuole | 3 |
| CrMTP2 | 153887 | 11 | 568 | Vacuole | 4 |
| CrMTP3 | 153892 | 11 | 318 | Organelle | – |
| CrMTP4 | 154037 | 11 | 295 | Vacuole | – |
| CrMTP5 | 162326 | 29 | 523 | – | 1 |
| ZIP Family | |||||
| CrZIP1 | 153077 | 10 | 300 | Organelle | – |
| CrZIP2 | 157106 | 167 | 745 | Vacuole | 1 |
| CrZIP3 | 160009 | 21 | 310 | Secretory pathway | – |
| CrZIP4 | 171817 | 98 | 334 | – | – |
| CrZIP5 | 171818 | 98 | 428 | Vacuole | 4 |
| CrZIP6 | 158835 | 19 | 413 | Vacuole | 3 |
| CrZIP7 | 167842 | 60 | 715 | – | 1 |
| CrZIP8 | 164608 | 3 | 294 | – | 2 |
| CrZIP9 | 153825 | 119 | 385 | Secretory pathway | 5 |
| CrZIP10 | 164248 | 38 | 289 | Vacuole | 1 |
| CrZIP11 | 164249 | 38 | 388 | Secretory pathway | 1 |
| CrZIP12 | 171130 | 8 | 480 | Secretory pathway | – |
| CrZIP13 putative | 159981 | 21 | 857 | Organelle | 2 |
| CrZIP14 putative | 164406 | 39 | 508 | Organelle | – |
| CAX Family | |||||
| CrCAX1 | 157233 | 16 | 447 | Vacuole | 11 |
| CrCAX2 | 166617 | 52 | 262 | Organelle | 3 |
| CrCAX3 | 158919 | 1 | 478 | Vacuole | – |
| CrCAX4 | 163085 | 31 | 709 | – | – |
| COPT Family | |||||
| CrCOPT1 | 163944 | 36 | 155 | – | – |
| P-Type ATPases | |||||
| HMA subfamily | |||||
| CrHMA1 | 159065 | 1 | 1,189 | Organelle | 1 |
| CrHMA2 | 168288 | 65 | 1,137 | – | 1 |
| CrHMA3 | 161566 | 26 | 937 | Vacuole | – |
| ABC Transporter Family | |||||
| MRP subfamily | |||||
| CrMRP1 | – | 61 | 1,082 | Secretory pathway | 21 |
| CrMRP2 | 153344 | 111 | 1,441 | Vacuole | 16 |
| CrMRP3 | 166481 | 51 | 1,519 | – | 2 |
| CrMRP4 | 165660 | 46 | 1,480 | Organelle | – |
| CrMRP5 | 162457 | 2 | 1,614 | – | – |
| CrMRP6 | – | 142 | 1,524 | – | 2 |
| CrMRP7 | 160938 | 24 | 875 | Vacuole | – |
| ATM/HMT subfamily | |||||
| CrCds1 | – | 122 | 1,062 | – | 7 |
| CrATM/HMT-2 | 158975 | 1 | 666 | Organelle | – |
| CrATM/HMT-3 | 156620 | 15 | 1,170 | – | – |
| FTR Family | |||||
| CrFTR1 | 164771 | 3 | 541 | Secretory pathway | 9 |
| NRAMP Family | |||||
| CrNRAMP1 | 157858 | 17 | 513 | Vacuole | 13 |
| CrNRAMP2 | 167153 | 57 | 287 | – | – |
| CrNRAMP3 | 165437 | 44 | 820 | Secretory pathway | – |