Figure 4.

Thermostability of CrNADP-MDH mutants in crude extracts. Reduced samples were first activated in the presence of 10 μm CrTRX m and 10 mm DTT for 10 min then heated at 45°C (A) and their activities measured. Oxidized samples (B–D) were first heated at the indicated temperatures for the indicated times, then cooled down and activated in the presence of 10 μm CrTRX m and 10 mm DTT for 10 min before activity measurements. Wild type, black circles and straight line; C330A, white circles and dotted line; C356A, black triangles and dashed line; C330A/C356A, white triangles, dashed and dotted line.