Table II.
Molecular features of some insertional mutants
Data relative to the positions, gene prediction, and homologies of the loci affected are described based on version 2 of the JGI Chlamydomonas genome server. Localizations of the insertions are described as follows: first in bold, numbers of scaffold and gene ID (when predicted by JGI) separated by two dots; second in parentheses, the scaffold position of the insertion with ← or → symbols indicating left or right direction of the vector, respectively; and, finally, the linkage group of the scaffold (when known) in roman numerals. GenBank NCBI accession numbers of the genomic sequence that flanked the DNA marker integrations are indicated in parenthesis below the names of mutants. Additional information about the real or putative locus affected is given just for those that are interrupted by the insertion. Unknown protein refers to putative predicted genes.
| Mutant
|
Insertion Localization
|
Putative Genes Affected
|
Notes
|
|
|---|---|---|---|---|
| Description | Biological Function | |||
| ON Phenotype | ||||
| 7.91 (AY704181 and AY704182) | 2 (←25,100) and 801:C_8010002 (←5,710) | Cnx2 | MoCo biosynthesis.Nitrate metabolism | Insertion located at 1.5 kb of Cnx2 |
| NI Phenotype | ||||
| 177.1 (AY704202) | 86 (109200→)/III | Nit2 | Major nitrate regulatory gene | Insertion located around 2.5 kb of Nit2 gene |
| 257.67 (AY704210) | 2458:C_24580001 (274→)/III | Nit2 | Major nitrate regulatory gene | In v.1 of JGI, insertion located around 15 kb of Nit2 gene (scaffold 17) |
| AI Phenotype | ||||
| 13.10 (AY704183) | 3 (243,560→)/III | EST presence | Same region as 22.82 | |
| 17.23 (AY704188) | 34 (←389,800) | EST presence | ||
| 19.32 (AY704184) | 16 (←276,000)/XII | EST presence | ||
| 20.40 (AY704185) | 6:C_60145 (←1,340,280)/X–XVIII | Unknown protein (EST presence) | ||
| 20.73 (AY704186) | 65:C_650021 (291,300→) | Homology with human guanylate cyclase (NP000848; 18,2% identity) | Intracellular signaling cascade | |
| 21.74 (AY704187) | 12:C_120097 (941,780→) | Homology with Chlamydomonas protein kinase (AAF97501; 20% identity) | Signal transduction mechanisms | |
| 21.88 (AY704189) | 115:C_1150005 (←125,000) | Dhc1 | Cytoskeleton | |
| 21.90 (AY704190) | 73 (←214,370)/IX | |||
| 22.82 (AY704191) | 3 (←240,890)/III | Same region as 13.10 | ||
| 25.38 (AY704192) | 291 (6500→) | Entire sequence not available in JGI | ||
| 42.49 (AY704193) | 6:C_60013 (←1,139,060)/X–XVIII | Unknown protein (EST presence) | ||
| 47.72 (AY704194) | 140:C_1400012 (201,650→) and 2079 (2870→) | Homology with human hsa:6421 SFPQ; splicing factor Pro/Gln rich (NP005057; 29% identity) | mRNA maturing | Two scaffolds with very similar sequences |
| 54.10 (AY704195) | 48 (585,760→)/XI | EST presence | Same region as 242.44 | |
| 85.37 (AY704196) | 49:C_490059 (79,700→)/XII | Protein with DNA-binding motif (BRIGHT/ARID domain) | DNA binding | Same locus as 255.92 mutant |
| 106.13 (AY704197) | 112:C_1120009 (196,360→) | Homology with EBNA-1 nuclear protein of human herpesvirus 4 (P03211; 18% identity) | DNA binding | |
| 106.20 (AY704198) | 48:C_480037 (560,410→)/XI | Homology with Fkb2 of Arabidopsis; PPIase, FKBP-type, rotamase (Q38935; 52% identity) | Protein folding: posttranslational modification, protein turnover, chaperones | Region very close to those of 54.10 and 242.44 mutants. This mutant has also an ON phenotype |
| 146.43 (AY704200) | 92:C_920053 (←384,230) | Ars | Endogenous Ars gene promoter is affected | |
| 169.84 (AY704201) | 11 (←580,870)/III | |||
| 207.31 (AY704203) | 80:C_800034 (←418,580 or ←425,460)/X | Unknown protein (EST presence) | Repetitive sequence in the same scaffold | |
| 209.34 (AY704204) | Unknown | No sequence available in JGI | ||
| 209.82 (AY704205) | 21:C_210095 and C_210005 (←1,044,850)/IX–XIV | Unknown protein | ||
| 213.94 (AY704206) | 2: C_20351 (←743,360)/II | Unknown protein (EST presence) | ||
| 219.8 (AY704207) | 6:C_60085 (699,600→)/X–XVIII | Homology with putative plant mitochondrial carrier proteins (EST presence) | Mitochondrial substrate carrier | |
| 242.44 (AY704208) | 48:C_480041 (589,690→)/XI | Unknown protein | Same region as 54.10 mutant | |
| 255.92 (AY704209) | 49:C_490059 (←82,420)/XII | Protein with DNA-binding motif (BRIGHT/ARID domain) | DNA binding | Same locus as 85.37 mutant |
| 258.90 (AY704211) | 156:C_1560006 (←15,250) | Homology with a PPIase, cyclophilin type of Arabidopsis (Q42406; 46% identity) | Protein folding: posttranslational modification, protein turnover, chaperones | |
| 259.89 (AY704212) | 35:C_350113 (623,140→)/I | Homology with ubiquitin-protein ligase genes | Protein modification, ubiquitin cycle | |