Table II.
Phenotypic description, flanking sequence data (by similarity to the Chlamydomonas genome sequence), and results of molecular analysis of insertional mutants
| Mutant ID | Phenotype(s) | Genome Position and Candidate Gene(s)a | No. of ble Insertionsb |
|---|---|---|---|
| CAL005.01.01 | Bleaches on HS | 185: 23950–23500 | n.d. |
| CAL005.01.13 | Acetate requiring, LL sensitive | 248: 49881–50798 | 1 |
| Genie 248.7 RBCS1 (Chlamydomonas) | |||
| Genie 248.8 RBCS2 (Chlamydomonas) | |||
| CAL005.01.20 | RB sensitive | 1725: 13500–13889 | 1 |
| CAL005.01.21 | LL sensitive, acetate requiring | 2187: 552–627 | 1 |
| CAL005.01.26 | LL sensitive, acetate requiring | 248: 14045–13965 | 1 |
| CAL007.01.01 | Bleaches at LL, RB, and MZ sensitive, low chlorophyll fluorescence | 86: 72908–73019 | 1 |
| Genie 86.13: β-7 subunit of 20S proteasome (rice) | |||
| Genie 86.14: Histone-binding protein N1/N2 (Xenopus laevis) | |||
| CAL007.01.04 | RB sensitive | 1243: 2407–2199 | 1 |
| Genie 1243.1 and 1243.2: Trans-splicing factor Raa3 (Chlamydomonas) | |||
| CAL007.01.09 | Yellow in the dark and at VLL | 137: 48662–48363 | 2 |
| Genewise 137.14.1: crtH; carotene isomerase (Synechocystis sp. PCC6803) | |||
| CAL007.01.11 | Bleaches on HS, MZ sensitive | 125: 47270–46956 | |
| Genewise 125.48.1: hemD, uroporphyrin III-synthase (Synechocystis sp. PCC6803) | |||
| CAL007.01.17 | Bleaches on MZ/HS | 45: 114609–114355 | 1 |
| CAL007.01.18 | Slight RB sensitivity | 876: 16393–16434 | 1 |
| Genie 876.2: Acetyl CoA synthetase (Arabidopsis) | |||
| CAL007.01.24 | LL sensitive, acetate requiring, high chlorophyll fluorescence | No genome similarity | 1 |
| CAL007.01.25 | Slight RB sensitivity | 1543: 7155–6541 | 1 |
| CAL007.01.26 | Acetate requiring, MZ sensitive | 416: 20998–20655 | 2 |
| CAL007.01.29 | Slight acetate requirement | No genome similarity | 1 |
| Identity to EST: 1031030D08.y1 | |||
| CAL007.01.30 | HL sensitive, acetate requiring, MZ and RB sensitive | Multiple hits, repeat region | 2 |
| CAL007.01.40 | RB sensitive | 387: 43424–43184 | 1 |
| CAL007.01.42 | RB sensitive, slight MZ sensitivity | 785: 20041–19556 | 1 |
| Genie 785.4: HSP101 (Arabidopsis) | |||
| CAL007.01.43 | MZ sensitive, some RB sensitivity | 239: 60731–60473 | 1 |
| Genie 239.1.1 Histone H2A (Chlamydomonas) | |||
| Genie 239.37.1 Histone H3 (Volvox carteri) | |||
| Genie 239.7.1 Histone H4 (Chlamydomonas) | |||
| CAL007.01.46 | Slight RB sensitivity | 119: 81484–81721 | 3 |
| CAL007.02.02 | LL sensitive, acetate requiring, partially rescued by high-CO2, low-chlorophyll content | 595: 18007–17767 | 1 |
| CAL007.02.03 | LL sensitive, acetate requiring | 1152: 5548–5360 | 3-5 |
| Genie 1152.2 ATP-synthase δ-chain (Chlamydomonas) | |||
| CAL007.02.09 | RB sensitive, reduced pigment at high CO2 | 91: 50935–50800 | 1 |
| Genie 91.10: ODA1 outer dynein arm docking protein (Chlamydomonas) | |||
| Genie 91.11 Silencing-related Ser-Thr kinase (rice) | |||
| CAL007.02.10 | MZ sensitive | 563: 15107–14707 | 1 |
| Genie 563.3 Digalactosyldiacylglycerol synthase (Arabidopsis) | |||
| CAL007.02.18 | Slight RB and MZ sensitivity | 1535: 12114–12248 | n.d. |
| CAL007.02.19 | Slight RB and MZ sensitivity | 851: 9886–9587 | n.d. |
| Genewise 851.5.1: Putative Cu(II)-type ascorbate-dependent monooxygenase (Arabidopsis) | |||
| CAL007.02.21 | Acetate requiring, HL sensitive, rescued by high CO2, RB sensitive, high chlorophyll fluorescence | Multiple hits in genome, repeat region | 1 |
| CAL007.02.27 | MZ sensitive, low growth rate | 89: 98986–99143 | 1 |
| Genie 89.16: Potential Cu-transporting ATPase type 3 (Arabidopsis) | |||
| Genie 89.17: Glutathione-requiring prostaglandin D-synthase (Gallus gallus) | |||
| CAL007.02.31 | Bleaches on MZ/HS | 65: 19631–20008 | 3 |
| Genie 65.2: Putative replication factor (Arabidopsis) | |||
| CAL007.02.38 | Acetate requiring | 1700: 10826–11047 | 1 |
| Genie 1700.6: Putative NADP oxidase (Vibrio cholerae) | |||
| Genie 1700.2–1700.5 Histone cluster (H3, H4, H2A, H2B-IV) | |||
| Genie 1700.1: Phosphoglycolate phosphatase chloroplast precursor (Chlamydomonas) | |||
| CAL007.02.47 | Slight MZ sensitivity | 68: 17490–17867 | 2 |
| Genie 68.3: Protein phosphatase 2C ABI1 (Arabidopsis) | |||
| CAL007.03.02 | RB sensitive | 1380: 4193–4169 | 2 |
| CAL007.03.03 | HL sensitive, RB sensitive | Multiple hits in genome, repeat region | 1 |
| CAL007.03.08 | Slight MZ sensitivity | 2640: 5102–5626 | 2 |
| Genie 2640.0: 70-kD heat shock protein (Chlamydomonas) | |||
| CAL007.03.10 | MZ sensitive | Multiple hits in genome, repeat region | 2 |
| CAL007.03.21 | Acetate requiring | 590: 28275–27677 | 1 |
| Genie 590.2 and 590.3: repair endonuclease (Arabidopsis) | |||
| CAL007.03.22 | MZ sensitive, slight RB sensitivity | 276: 20397–20609 | 1 |
| Genewise.276.32.1 Dynein 11-kD light chain flagellar outer arm (Chlamydomonas) | |||
| Genie 276.2 cgcr-4 protein (Chlamydomonas) | |||
| CAL007.03.26 | MZ sensitive | Fragment 1: multiple hits, repeat region | 1 |
| Fragment 2: 228: 4384–4683 | |||
| CAL007.03.32 | Acetate requiring, HL sensitive, rescued by high CO2 | 3868: 836–879 | 2 |
| CAL007.03.34 | HL sensitive, partially acetate requiring, rescued by high CO2 | 899: 9585–10323 | 3 |
| CAL007.03.41 | Acetate requiring, RB sensitive | Fragment 1: 199: 124–58 | 4 |
| Genie 199.1: phosphoenolpyruvate-dependent sugar phosphotransferase system | |||
| Fragment 2: 901: 31035–30595 | |||
| CAL007.03.43 | MZ sensitive | 248: 42663–48811 (discontinuous) | 1 |
| Genie 248.8: RBCS2 | |||
| CAL007.03.45 | Acetate requiring | 3: 156677–156894 | 2 |
| CAL007.03.46 | Acetate requiring, partially rescued by high CO2 | Multiple hits, repeat region | 2 |
| CAL007.03.47 | Acetate requiring, HL sensitive | 45: 151884–152295 | 1 |
| CAL010.01.02 | RB sensitive | 732: 12958–12763 | 1 |
| Genie 732.2 Autolysin (gametolysin) precursor (Chlamydomonas) | |||
| CAL010.01.10 | RB sensitive, pale green | 1214: 1678–1021 (discontinuous) | 1 |
| CAL010.01.11 | LL sensitive | 62: 19180–19068 | 1 |
| Genie 62.4 | |||
| Genie 62.5 Succinate dehydrogenase (ubiquinone) iron-sulfur protein precursor (Drosophila) | |||
| CAL010.01.21 | RB sensitive, MZ sensitive | 23: 50197–50354 | 1 |
| CAL010.01.31 | RB sensitive | 102: 2403–2506 | n.d. |
| Genewise 102.30.1: Calmodulin-binding protein (Arabidopsis) |
a
Determined by comparison with the Chlamydomonas nuclear genome sequence, version 1.0 (http://genome.jgi-psf.org/chlre1/chlre1.home.html). Alignment of the flanking sequence with the genome sequence is indicated by scaffold number (in bold) followed by sequence range in base pairs.
b
Number of ble insertions determined by DNA gel-blot analysis. n.d., Not determined.