Table II.

Concentration (copies per cell) of the 14 spike RNAs for the seven different spike mixes and the reference mix^a

Each spike RNA was calibrated and mixed in equal amount with one of the other spike RNAs to obtain seven pairs at equal concentration (labeled a–g).

Spike No.	Spike Mix 1	Spike Mix 2	Spike Mix 3	Spike Mix 4	Spike Mix 5	Spike Mix 6	Spike Mix 7	Reference Mix
1, 8 (a)	10,000	0	0.1	1	10	100	1,000	100
2, 9 (b)	1,000	10,000	0	0.1	1	10	100	100
3, 10 (c)	100	1,000	10,000	0	0.1	1	10	100
4, 11 (d)	10	100	1,000	10,000	0	0.1	1	100
5, 12 (e)	1	10	100	1,000	10,000	0	0.1	100
6, 13b (f)	0.1	1	10	100	1,000	10,000	0	100
7, 14 (g)	0	0.1	1	10	100	1,000	10,000	100

^aMore detailed information on the spiked RNAs can be found in Supplemental Table II.

^bSpike 13 was eliminated from further analysis because its quality was insufficient.