Table V.
Total leaf and BS Chl and N, thylakoid N, soluble protein, amino acids, nitrate, Rubisco, PSII, PSI, and Cyt f concentrations of two NAD-ME (P. miliaceum and P. coloratum) and two NADP-ME (S. bicolor and C. ciliaris) C4 grasses (experiment 4; Table I)
Dark respiration (Rd) and dark-adapted Fv/Fm are also shown. Values are means ± se. Significance levels are as described in the legend of Figure 1. Within the same row, values followed by the same letter are not significantly different (P > 0.05).
| Parameter | Fraction | P. miliaceum | P. coloratum | S. bicolor | C. ciliaris |
|---|---|---|---|---|---|
| Chl a/b | Leaf (n = 12) | 4.2a ± 0.1 | 4.3ab ± 0.1 | 4.4b ± 0.1 | 4.4ab ± 0.1 |
| BS (n = 4) | 4.1a ± 0.1 | 4.1a ± 0.2 | 6.3b ± 0.3 | 6.4b ± 0.5 | |
| PFraction | ns | ns | ** | ** | |
| Chl (μmol m−2) | Leaf (n = 12) | 424a ± 26 | 425a ± 24 | 579b ± 36 | 464a ± 37 |
| BS (n = 4) | 284b ± 18 | 256b ± 26 | 206a ± 15 | 164a ± 21 | |
| BS/Leaf (PFraction) | 0.60 (*) | 0.59 (*) | 0.33 (**) | 0.38 (**) | |
| Total N (mmol m−2) | Leaf (n = 6) | 78a ± 10 | 87a ± 8 | 116b ± 3 | 87a ± 3 |
| BS (n = 3) | 47b ± 5 | 51b ± 3 | 39ab ± 4 | 33a ± 2 | |
| BS/Leaf (PFraction) | 0.67 (*) | 0.60 (***) | 0.36 (***) | 0.35 (**) | |
| Thylakoid N (mmol N m−2) | Leaf (n = 3) | 23.5a ± 1.7 | 25.2ab ± 0.4 | 30.3c ± 0.5 | 28.6bc ± 0.5 |
| BS (n = 2) | 14.0c ± 0.3 | 12.0b ± 0.7 | 11.2b ± 0.3 | 9.2a ± 0.3 | |
| BS/Leaf (PFraction) | 0.60 (*) | 0.48 (**) | 0.37 (***) | 0.32 (**) | |
| Soluble protein (g m−2) | Leaf (n = 3) | 3.8a ± 0.4 | 4.6a ± 0.3 | 3.7a ± 0.4 | 3.4a ± 0.2 |
| BS (n = 3) | 2.3a ± 0.3 | 3.2a ± 1.1 | 2.0a ± 0.2 | 1.9a ± 0.1 | |
| BS/Leaf (PFraction) | 0.61 (*) | 0.64 (*) | 0.55 (*) | 0.55 (*) | |
| Amino acids (mmol m−2) | Leaf (n = 3) | 6.6a ± 0.6 | 6.1a ± 0.9 | 10.0a ± 1.7 | 5.7a ± 0.8 |
| BS (n = 2) | 1.3a ± 0.1 | 1.8a ± 0.5 | 1.3a ± 0.1 | 0.90a ± 0.1 | |
| BS/Leaf (PFraction) | 0.20 (**) | 0.30 (*) | 0.13 (*) | 0.16 (*) | |
| Nitrate (mmol m−2) | Leaf (n = 3) | 3.7a ± 0.1 | 4.6b ± 0.4 | 7.5bb ± 1.2 | 2.7a ± 0.4 |
| BS (n = 2) | 1.7a ± 0.3 | 2.1a ± 0.5 | 1.9a ± 0.1 | 1.3a ± 0.1 | |
| BS/Leaf (PFraction) | 0.46 (**) | 0.45 (*) | 0.25 (*) | 0.49 (*) | |
| Rubisco (μmol m−2) | Leaf (n = 12) | 0.97b ± 0.06 | 1.25c ± 0.08 | 0.97b ± 0.06 | 0.73a ± 0.04 |
| PSII (μmol m−2) | Leaf (n = 6) | 1.03a ± 0.06 | 0.99a ± 0.08 | 1.35b ± 0.06 | 1.15a ± 0.06 |
| BS (n = 2) | 0.18b ± 0.02 | 0.35c ± 0.01 | 0.02a ± 0.02 | 0.05a ± 0.01 | |
| BS/Leaf (PFraction) | 0.17 (***) | 0.35 (***) | 0.01 (***) | 0.04 (***) | |
| PSI (μmol m−2) | Leaf (n = 2) | 1.06a ± 0.07 | 0.95a ± 0.05 | 1.01a ± 0.01 | 0.93a ± 0.13 |
| BS (n = 2) | 0.26a ± 0.07 | 0.44b ± 0.02 | 0.37ab ± 0.01 | 0.36ab ± 0.03 | |
| BS/Leaf (PFraction) | 0.24 (*) | 0.46 (*) | 0.37 (***) | 0.39 (*) | |
| Cyt f (μmol m−2) | Leaf (n = 2) | 1.03a ± 0.02 | 1.00a ± 0.05 | 1.00a ± 0.04 | 1.05a ± 0.16 |
| BS (n = 2) | 0.39c ± 0.02 | 0.41c ± 0.03 | 0.20a ± 0.01 | 0.30b ± 0.01 | |
| BS/Leaf (PFraction) | 0.37 (**) | 0.38 (*) | 0.20 (**) | 0.34 (*) | |
| Rd (μmol m−2 s−1) | Leaf (n = 3) | −1.7a ± 0.12 | −1.9a ± 0.08 | −1.9a ± 0.10 | −1.8a ± 0.40 |
| Fv/Fm | Leaf (n = 3) | 0.75a ± 0.01 | 0.81a ± 0.01 | 0.80a ± 0.01 | 0.80a ± 0.01 |
| BS (n = 3) | 0.75b ± 0.01 | 0.81b ± 0.01 | 0.40a ± 0.01 | 0.45a ± 0.02 | |
| PFraction | ns | ns | *** | *** |