Figure 3.
Siliques of the Ler (wild type), crc-1 ET668, and PROCRC:CRC-INO transformants of crc-1 ET668. A, crc-1 ET668. B, Wild type. C, PROCRC:CcCI. D, PROCRC:IiIC. E, PROCRC:CiII. F, PROCRC:IcCC. G, PROCRC:IcCI. H, PROCRC:CiIC. I, PROCRC:INO. J, PROCRC:CRC. Chimeras with two CRC regions generally rescued carpel fusion but failed to rescue silique growth, with some having siliques broader than wild type. K, PROCRC:CRC fully complemented the crc-1 mutant with all nectaries, lateral and medial, present, as assayed by GUS staining in the nectary conferred by enhancer trap ET668 (arrowheads). L, Partial complementation, the development of lateral nectaries only, was observed using constructs that contained two of three CRC domains, e.g. PROCRC:CcCI. M, PROCRC:INO failed to complement crc-1, with no nectary development detected.