Figure 2.

Auxin induces NO accumulation. a and b, Soybean roots were loaded with 10 μm DAF-2DA for 30 min and then control buffer (A) or 5 μm IAA (B) applied via agar block to the left-hand side of the vertically orientated root. NO was imaged after 30 min. Scale bar = 1 mm. Experiments were repeated five times with similar results. c, Protoplasts were loaded with DAF-2DA and then incubated in 5 μm IAA for 30 min prior to flow cytometry. Experiments were repeated three times with similar results. d, Protoplasts were loaded with DAF-2DA and then incubated with 5 μm IAA in combination with 20 μm l-NAA, 20 μm NaN₃, or 50 μm cPTIO. Experiments were repeated three times with similar results.