Gravistimulation induces the rapid accumulation of cGMP in soybean roots. a, Kinetics of cGMP accumulation in Zone 1 (•) or Zone 2 (○) from horizontal roots following gravistimulation. ▾, Vertical roots. Values are the means ± se for five independent experiments. b, Asymmetric distribution of cGMP in root Zone 1. Soybean roots were gravistimulated for different times, bisected horizontally with a razor blade, and cGMP content assayed. ▾, Vertical roots; ○, lower half of horizontal root; •, upper half of horizontal root. c, Roots were pretreated as indicated for 12 h and then placed horizontally for 12 h. On x axis: CK, Control; G, gravistimulated. Data analyzed by one-way ANOVA followed by Tukey's test. Different symbols indicate significant differences between treatments (P < 0.05).