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. 2023 Sep 29;12:e85398. doi: 10.7554/eLife.85398

Figure 5. Excitatory and inhibitory pre-Bötzinger complex (preBötC) neurons are transduced by selective transduction of preBötC →7n neurons.

Figure 5.

(A) Schematic diagram showing the injection protocol for selective transduction of preBötC→7n neurons. (B) Quantification of the total number of MuGFP-expressing neurons, plotted as the distance from Bregma (mm). Note the slight trend toward a more caudal distribution. The histograms show the number of transduced cells that co-expressed mRNA for VGAT or VGluT2. The results are presented as mean ± 95% CI. (C–F) In situ hybridization showing the co-expression of MuGFP (green), with mRNA for VGAT (red), VGluT2 (magenta), reelin (cyan), or somatostatin (SST) (yellow) in preBötC→7n neurons. Nuclei are labeled in blue (DAPI). The yellow arrows highlight VGAT neurons, and the white arrows highlight VGluT2 neurons, the red arrows highlight reelin neurons, and the pink arrows highlight SST neurons. (G) Immunohistochemistry showing that some neurons expressing MuGFP (green) and mCherry (magenta) also express neurokinin-1 receptor (NK1R) (light blue). Co-localization is indicated by white arrows.